Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: Schematic diagram of the CpG sites in the maspin promoter. The promoter region and exon 1 of the maspin gene are shown. Vertical lines indicate the location of the CpG sites studied. Each CpG site is named according to its nucleotide position from the transcription start site (bent arrow). The locations of the primers used in the study are as indicated schematically. Details of the primer extension reaction are shown below the primers. The location of the -156 SNP is indicated in square brackets. The A/C SNP, when bisulfite-converted and interrogated in the reverse sense, becomes a T/A SNP. Nucleotide positions on the extension primer that correspond to the unmethylated CpG sites are indicated by the positions marked with an A (adenine). hME, homogenous MassEXTEND.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques:

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: Cloning and bisulfite sequencing of the maspin promoter in first- and third-trimester paired placental tissues and maternal blood cells. The CpG sites depicted across the first row are named with the same numbering system as in Fig. 1. Each subsequent row depicts the methylation status across the CpG sites in a single DNA molecule isolated by cloning. Filled and unfilled circles represent methylated and unmethylated CpG sites, respectively. Results for four cases are shown.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Cloning, Methylation Sequencing, Methylation, Isolation

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: Box plots of U-maspin concentrations in first-, second-, and third-trimester maternal plasma. The line within each box denotes the median. Limits of the box denote the 25th and 75th percentiles. Whiskers denote the 5th and 95th percentiles. Filled circles depict the outliers.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Clinical Proteomics

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: U-maspin (A), SRY (B), and M-maspin (C) concentrations in maternal plasma before and 24 h after delivery. Paired samples from the same pregnancy are depicted by identical symbols connected by a line.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Clinical Proteomics

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: Analysis of Maspin promoter −156 SNP genotype in maternal and fetal genomic and bisulfite-converted DNA in maternal plasma

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Clinical Proteomics

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: MS tracings of the maspin -156 SNP genotype in case 300. (Top and Middle) The maternal and fetal genotypes determined from genomic DNA of maternal blood cells and placental tissues, respectively, are shown. (Lower) The U-maspin genotype detected in the corresponding bisulfite converted maternal plasma samples are shown. For all mass spectra, the x axis depicts the molecular weight of the detected extension products (shown as sharp peaks), whereas the y axis depicts the intensity in arbitrary units. The expected positions of the A- and C-alleles are as marked. UEP, unextended primer.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Clinical Proteomics, Molecular Weight

Journal:

Article Title: Detection of the placental epigenetic signature of the maspin gene in maternal plasma

doi: 10.1073/pnas.0503335102

Figure Lengend Snippet: U-maspin concentrations in maternal plasma of women with preeclamptic (PET) and healthy (Normal) pregnancies. The line within each box denotes the median. Limits of the box denote the 25th and 75th percentiles. Whiskers denote the 5th and 95th percentiles. Filled circles depict the outliers.

Article Snippet: Concentrations of methylated (M- maspin ) and unmethylated (U- maspin ) maspin promoter DNA sequences were measured in maternal plasma by real-time quantitative methylation-specific PCR assays ( ) ( 24 ) on an Applied Biosystems 7900 HT Sequence Detection System.

Techniques: Clinical Proteomics

Journal: Neural Development

Article Title: A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire

doi: 10.1186/1749-8104-6-37

Figure Lengend Snippet: Genetic interaction of lola and spire mutations . Stage 17 embryos of the indicated genotypes were fixed and stained with anti-Fasciclin 2 to label ISNb motor axons, and visualized by peroxidase histochemistry. Three representative hemisegments are shown in each panel. (A) Wild type (WT). ISNb forms three neuromuscular junctions (NMJs) to ventral longitudinal muscles per hemisegment (arrows). (B) spir 1 homozygous mutant. NMJs are indicated with arrows, positions where NMJs are missing are highlighted with asterisks. (C) lola e76 /lola c46 null mutant. (D) lola e76 spir 1 /lola c46 embryo. Note partial restoration of NMJs. (E) Quantification of NMJ number in embryos of the indicated genotypes. Average number of NMJs per hemisegment is plotted as blue bars. Thin lines indicate standard error of the mean of three experiments (N = approximately 200 hemisegments per dataset).

Article Snippet: Primer sets were purchased commercially (Applied Biosystems) as follows: Ribosomal protein L32 (RPL32; Dm02151827_g1); Bekka (Dm02363268_s1); blistery (Dm02138346_m1); CG6070 (Dm02145281_m1); charybde (Dm01802349_m1); Kruppel target at 95D (Dm02150605_m1); Neural Lazarillo (Dm01844577_g1); spire (Dm01811138_m1); Target of rapamycin (Dm01843300_g1); and walrus (Dm01792969_g1).

Techniques: Staining, Muscles, Mutagenesis